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recombinant mouse il 9 protein  (R&D Systems)


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    Structured Review

    R&D Systems recombinant mouse il 9 protein
    Recombinant Mouse Il 9 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+il+9/Recombinant+Mouse+IL-9+Protein/pm39945866-127-7-11
    Average 93 stars, based on 16 article reviews
    recombinant mouse il 9 protein - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Cloning and functional testing of rhesus macaque ( Macaca mulatta ) IL-9 and IL-33
    Article Snippet: MO7e cells were incubated with rhesus IL-9 supernatant (culture supernatant of 1:10 dilution) and 30 μg/ml of IL-9 neutralizing antibody (cat # AB-209-NA, R&D systems) whereas D10.G4.T1 were treated with rhesus IL-33 supernatant (culture supernatant with no dilution) and 5 μg/ml of IL-33 neutralizing antibody (cat # MAB36254,R&D systems), incubated for 3 days at 37°C and 5% CO 2 and cell proliferation was measured by cell count using acridine orange and propidium iodide staining and a LUNA-FL automated cell counter (Logos Biosystems). .. 20 ng/ml of human recombinant IL-9 (cat # 209-ILB/CF, R&D systems) and 20 ng/ml of IL-33 (cat #200-33, Peprotech) were used as positive controls and pUNO-1 empty vector transfected cell supernatant (no dilution) was used as a negative control to treat the MO7e and D10.G4.T1. ..

    Article Title: Cloning and functional testing of rhesus macaque (Macaca mulatta) IL-9 and IL-33.
    Article Snippet: MO7e cells were incubated with rhesus IL-9 supernatant (culture supernatant of 1:10 dilution) and 30 μg/ mL of IL-9 neutralizing antibody (cat # AB-209-NA, R&D systems) whereas D10.G4.T1 was treated with rhesus IL-33 supernatant (culture supernatant with no dilution) and 5 μg/mL of IL-33 neutralizing antibody (cat # MAB36254, R&D systems), incubated for 3 days at 37°C and 5% CO2, and cell proliferation was measured by cell count using acridine orange and propidium iodide staining and a LUNA-FL automated cell counter (Logos Biosystems). .. Twenty ng/ml of human recombinant IL-9 (cat # 209-ILB/CF, R&D systems) and 20 ng/mL of IL-33 (cat #200-33, Peprotech) were used as positive controls, and pUNO-1 empty vector-transfected cell supernatant (no dilution) was used as a negative control to treat the MO7e and D10.G4.T1. .. Similarly, rhesus BLCL-C162 cells (NHP Reagent Resource) were treated with rhesus IL-9 (no dilution) and IL-33 (no dilution) supernatant, incubated for 3 days at 37°C 5% CO2, and cell proliferation was measured by cell count as mentioned previously.

    Article Title: IL-9 Is a Biomarker of BIA-ALCL Detected Rapidly by Lateral Flow Assay.
    Article Snippet: .. Recombinant IL-9 (R&D Systems, catalog number 209-ILB/CF) or CD30 (R&D Systems, catalog number 844155) proteins ranging from 0 to 50 ng/mL were diluted in the benign seroma and also tested on the strips as standards. ..

    Plasmid Preparation:

    Article Title: Cloning and functional testing of rhesus macaque ( Macaca mulatta ) IL-9 and IL-33
    Article Snippet: MO7e cells were incubated with rhesus IL-9 supernatant (culture supernatant of 1:10 dilution) and 30 μg/ml of IL-9 neutralizing antibody (cat # AB-209-NA, R&D systems) whereas D10.G4.T1 were treated with rhesus IL-33 supernatant (culture supernatant with no dilution) and 5 μg/ml of IL-33 neutralizing antibody (cat # MAB36254,R&D systems), incubated for 3 days at 37°C and 5% CO 2 and cell proliferation was measured by cell count using acridine orange and propidium iodide staining and a LUNA-FL automated cell counter (Logos Biosystems). .. 20 ng/ml of human recombinant IL-9 (cat # 209-ILB/CF, R&D systems) and 20 ng/ml of IL-33 (cat #200-33, Peprotech) were used as positive controls and pUNO-1 empty vector transfected cell supernatant (no dilution) was used as a negative control to treat the MO7e and D10.G4.T1. ..

    Transfection:

    Article Title: Cloning and functional testing of rhesus macaque ( Macaca mulatta ) IL-9 and IL-33
    Article Snippet: MO7e cells were incubated with rhesus IL-9 supernatant (culture supernatant of 1:10 dilution) and 30 μg/ml of IL-9 neutralizing antibody (cat # AB-209-NA, R&D systems) whereas D10.G4.T1 were treated with rhesus IL-33 supernatant (culture supernatant with no dilution) and 5 μg/ml of IL-33 neutralizing antibody (cat # MAB36254,R&D systems), incubated for 3 days at 37°C and 5% CO 2 and cell proliferation was measured by cell count using acridine orange and propidium iodide staining and a LUNA-FL automated cell counter (Logos Biosystems). .. 20 ng/ml of human recombinant IL-9 (cat # 209-ILB/CF, R&D systems) and 20 ng/ml of IL-33 (cat #200-33, Peprotech) were used as positive controls and pUNO-1 empty vector transfected cell supernatant (no dilution) was used as a negative control to treat the MO7e and D10.G4.T1. ..

    Negative Control:

    Article Title: Cloning and functional testing of rhesus macaque ( Macaca mulatta ) IL-9 and IL-33
    Article Snippet: MO7e cells were incubated with rhesus IL-9 supernatant (culture supernatant of 1:10 dilution) and 30 μg/ml of IL-9 neutralizing antibody (cat # AB-209-NA, R&D systems) whereas D10.G4.T1 were treated with rhesus IL-33 supernatant (culture supernatant with no dilution) and 5 μg/ml of IL-33 neutralizing antibody (cat # MAB36254,R&D systems), incubated for 3 days at 37°C and 5% CO 2 and cell proliferation was measured by cell count using acridine orange and propidium iodide staining and a LUNA-FL automated cell counter (Logos Biosystems). .. 20 ng/ml of human recombinant IL-9 (cat # 209-ILB/CF, R&D systems) and 20 ng/ml of IL-33 (cat #200-33, Peprotech) were used as positive controls and pUNO-1 empty vector transfected cell supernatant (no dilution) was used as a negative control to treat the MO7e and D10.G4.T1. ..

    Article Title: Cloning and functional testing of rhesus macaque (Macaca mulatta) IL-9 and IL-33.
    Article Snippet: MO7e cells were incubated with rhesus IL-9 supernatant (culture supernatant of 1:10 dilution) and 30 μg/ mL of IL-9 neutralizing antibody (cat # AB-209-NA, R&D systems) whereas D10.G4.T1 was treated with rhesus IL-33 supernatant (culture supernatant with no dilution) and 5 μg/mL of IL-33 neutralizing antibody (cat # MAB36254, R&D systems), incubated for 3 days at 37°C and 5% CO2, and cell proliferation was measured by cell count using acridine orange and propidium iodide staining and a LUNA-FL automated cell counter (Logos Biosystems). .. Twenty ng/ml of human recombinant IL-9 (cat # 209-ILB/CF, R&D systems) and 20 ng/mL of IL-33 (cat #200-33, Peprotech) were used as positive controls, and pUNO-1 empty vector-transfected cell supernatant (no dilution) was used as a negative control to treat the MO7e and D10.G4.T1. .. Similarly, rhesus BLCL-C162 cells (NHP Reagent Resource) were treated with rhesus IL-9 (no dilution) and IL-33 (no dilution) supernatant, incubated for 3 days at 37°C 5% CO2, and cell proliferation was measured by cell count as mentioned previously.



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    Journal: Cell reports

    Article Title: MicroRNA-19b exacerbates systemic sclerosis through promoting Th9 cells

    doi: 10.1016/j.celrep.2024.114565

    Figure Lengend Snippet:

    Article Snippet: Recombinant Mouse IL-9 , R&D Systems , Cat# 409-ML.

    Techniques: Purification, Control, Virus, Plasmid Preparation, Expressing, Retroviral, Recombinant, Activation Assay, Modification, Infection, Transfection, Lysis, Extraction, Western Blot, Electron Microscopy, Cell Isolation, Staining, Mutagenesis, Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Gene Expression, cDNA Synthesis, Sequencing, Software, Real-time Polymerase Chain Reaction